Journal article
Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA) Samples from Advanced Non-Small Cell Lung Cancer for Whole Genome, Whole Exome and Comprehensive Panel Sequencing
D Fielding, V Lakis, AJ Dalley, H Chittoory, F Newell, LT Koufariotis, AM Patch, S Kazakoff, F Bashirzadeh, JH Son, K Ryan, D Steinfort, JP Williamson, M Bint, C Pahoff, PT Nguyen, S Twaddell, D Arnold, C Grainge, A Pattison Show all
Cancers | Published : 2024
Abstract
Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is often the only source of tumor tissue from patients with advanced, inoperable lung cancer. EBUS-TBNA aspirates are used for the diagnosis, staging, and genomic testing to inform therapy options. Here we extracted DNA and RNA from 220 EBUS-TBNA aspirates to evaluate their suitability for whole genome (WGS), whole exome (WES), and comprehensive panel sequencing. For a subset of 40 cases, the same nucleic acid extraction was sequenced using WGS, WES, and the TruSight Oncology 500 assay. Genomic features were compared between sequencing platforms and compared with those reported by clinical testing. A total of 204 as..
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Grants
Awarded by Cooperative Research Centres, Australian Government Department of Industry
Funding Acknowledgements
We thank all patients that have taken part of our study in such difficult time in their lives and all nursing staff across our bronchoscopy departments and Elizabeth Newland for their support recruiting patients. We also sincerely thank Mahendra Singh, Lakshmy Nandakumar and Kaltin Ferguson for their continued support in this program of work regarding diagnostic pathology review and sample processing, respectively.